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This research was supported by FCT (Portuguese Foundation for Science and Technology), through the grants attributed to D Crespo (SFRH/BD/80252/2011), T F Grilo (SFRH/BPD/98590/2013), J P Coelho (SFRH/ BPD/102870/2014), I Fernandes (SFRH/BPD/97656/2013), to Investigador FCT programme attributed to M. Dolbeth (IF/00919/2015) and BIOCHANGED project (PTDC/MAR/111901/2009), subsidized by the European Social Fund and MCTES (Portuguese Ministry of Science, Technology and Higher Education), through the POPH (Human Potential Operational Programme), QREN (National Strategic Reference Framework) and COMPETE (Programa Operacional Factores de Competitividade). Thanks are also due, for the financial support to CESAM (UID/AMB/50017/2013), to FCT/MEC through national funds, and the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020.