Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/101264
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rebelo, Catarina | - |
dc.contributor.author | Reis, Tiago | - |
dc.contributor.author | Guedes, Joana | - |
dc.contributor.author | Saraiva, Cláudia | - |
dc.contributor.author | Rodrigues, Artur Filipe | - |
dc.contributor.author | Simões, Susana | - |
dc.contributor.author | Bernardino, Liliana | - |
dc.contributor.author | Peça, João | - |
dc.contributor.author | Pinho, Sónia L. C. | - |
dc.contributor.author | Ferreira, Lino | - |
dc.date.accessioned | 2022-08-18T11:10:37Z | - |
dc.date.available | 2022-08-18T11:10:37Z | - |
dc.date.issued | 2022 | - |
dc.identifier.issn | 2041-1723 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/101264 | - |
dc.description.abstract | Spatial control of gene expression is critical to modulate cellular functions and deconstruct the function of individual genes in biological processes. Light-responsive gene-editing formulations have been recently developed; however, they have shown limited applicability in vivo due to poor tissue penetration, limited cellular transfection and the difficulty in evaluating the activity of the edited cells. Here, we report a formulation composed of upconversion nanoparticles conjugated with Cre recombinase enzyme through a photocleavable linker, and a lysosomotropic agent that facilitates endolysosomal escape. This formulation allows in vitro spatial control in gene editing after activation with near-infrared light. We further demonstrate the potential of this formulation in vivo through three different paradigms: (i) gene editing in neurogenic niches, (ii) gene editing in the ventral tegmental area to facilitate monitoring of edited cells by precise optogenetic control of reward and reinforcement, and (iii) gene editing in a localized brain region via a noninvasive administration route (i.e., intranasal). | pt |
dc.language.iso | eng | pt |
dc.publisher | Nature | pt |
dc.relation | POCI-01-0145-FEDER-016390:CANCEL STEM | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC/NAN-MAT/28060/2017/PT/BrainEdition: remotely controlling gene edition in neural stem cells | pt |
dc.relation | POCI-01-0145- FEDER-016682 | pt |
dc.relation | info:eu-repo/grantAgreement/CENTRO-01-0145-FEDER-000014/PT | pt |
dc.relation | Interreg 2IQBioNeuro | pt |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/669088/EU/Enhancing Research in Ageing at the University of Coimbra | pt |
dc.relation | EAPA_791/2018 - NEUROATLANTIC entitled: ‘An Atlantic innovation platform on diagnosis and treatment of neurological diseases and aging’ | pt |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/952266/EU/RESEarch for healThy AGEING | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/OE/PD/BD/52337/2013/PT/Nanomedicine to enhance heart regenerative potential | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH/BPD/96048/2013/PT/Cellular reprogramming by near infrared NIR-activatable nanoparticles containing transcription factors - NanoTarget: remote modulation for cardiac progenitor cells | pt |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/101003413/EU/LIght-responsive GrapHene-based inTerfaces for Electrical STimulation | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Brain | pt |
dc.subject.mesh | Infrared Rays | pt |
dc.subject.mesh | Optogenetics | pt |
dc.subject.mesh | Proteins | pt |
dc.subject.mesh | Gene Editing | pt |
dc.subject.mesh | Nanoparticles | pt |
dc.title | Efficient spatially targeted gene editing using a near-infrared activatable protein-conjugated nanoparticle for brain applications | pt |
dc.type | article | - |
degois.publication.firstPage | 4135 | pt |
degois.publication.issue | 1 | pt |
degois.publication.title | Nature Communications | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1038/s41467-022-31791-6 | pt |
degois.publication.volume | 13 | pt |
dc.date.embargo | 2022-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | RESEarch for healThy AGEING | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-4078-3455 | - |
crisitem.author.orcid | 0000-0003-4989-2129 | - |
crisitem.author.orcid | 0000-0001-8985-9302 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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s41467-022-31791-6.pdf | 2.23 MB | Adobe PDF | View/Open |
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