Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/103874
DC Field | Value | Language |
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dc.contributor.author | Tawfik, Bassam | - |
dc.contributor.author | Martins, Joana S | - |
dc.contributor.author | Houy, Sébastien | - |
dc.contributor.author | Imig, Cordelia | - |
dc.contributor.author | Pinheiro, Paulo S. | - |
dc.contributor.author | Wojcik, Sonja M | - |
dc.contributor.author | Brose, Nils | - |
dc.contributor.author | Cooper, Benjamin H | - |
dc.contributor.author | Sørensen, Jakob Balslev | - |
dc.date.accessioned | 2022-12-06T10:46:29Z | - |
dc.date.available | 2022-12-06T10:46:29Z | - |
dc.date.issued | 2021-03-22 | - |
dc.identifier.issn | 2050-084X | pt |
dc.identifier.uri | https://hdl.handle.net/10316/103874 | - |
dc.description.abstract | Synaptotagmins confer calcium-dependence to the exocytosis of secretory vesicles, but how coexpressed synaptotagmins interact remains unclear. We find that synaptotagmin-1 and synaptotagmin-7 when present alone act as standalone fast and slow Ca2+-sensors for vesicle fusion in mouse chromaffin cells. When present together, synaptotagmin-1 and synaptotagmin-7 are found in largely non-overlapping clusters on dense-core vesicles. Synaptotagmin-7 stimulates Ca2+-dependent vesicle priming and inhibits depriming, and it promotes ubMunc13-2- and phorbolester-dependent priming, especially at low resting calcium concentrations. The priming effect of synaptotagmin-7 increases the number of vesicles fusing via synaptotagmin-1, while negatively affecting their fusion speed, indicating both synergistic and competitive interactions between synaptotagmins. Synaptotagmin-7 places vesicles in close membrane apposition (<6 nm); without it, vesicles accumulate out of reach of the fusion complex (20-40 nm). We suggest that a synaptotagmin-7-dependent movement toward the membrane is involved in Munc13-2/phorbolester/Ca2+-dependent priming as a prelude to fast and slow exocytosis triggering. | pt |
dc.language.iso | eng | pt |
dc.publisher | eLife Sciences Publications | pt |
dc.relation | University of Copenhagen KU2016 | pt |
dc.relation | Novo Nordisk Foundation NNF19OC0058298 | pt |
dc.relation | Lundbeckfonden R221-2016-1202 | pt |
dc.relation | Independent Research Fund Denmark 0134-00141A | pt |
dc.relation | Lundbeckfonden R34-A3740 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | SNARE-proteins; capacitance measurements; chromaffin cell; mouse; neuroscience; neurotransmitter release; synaptotagmin-7; vesicle priming | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Calcium | pt |
dc.subject.mesh | Cell Membrane | pt |
dc.subject.mesh | Chromaffin Cells | pt |
dc.subject.mesh | Electron Microscope Tomography | pt |
dc.subject.mesh | Exocytosis | pt |
dc.subject.mesh | Intracellular Signaling Peptides and Proteins | pt |
dc.subject.mesh | Membrane Fusion | pt |
dc.subject.mesh | Mice | pt |
dc.subject.mesh | Mice, Inbred C57BL | pt |
dc.subject.mesh | Nerve Tissue Proteins | pt |
dc.subject.mesh | Secretory Vesicles | pt |
dc.subject.mesh | Synaptotagmins | pt |
dc.title | Synaptotagmin-7 places dense-core vesicles at the cell membrane to promote Munc13-2- and Ca2+-dependent priming | pt |
dc.type | article | - |
degois.publication.firstPage | e64527 | pt |
degois.publication.title | eLife | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.7554/eLife.64527 | pt |
degois.publication.volume | 10 | pt |
dc.date.embargo | 2021-03-22 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-9024-1021 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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elife-64527-v2.pdf | 4.84 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License