Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/106654
DC Field | Value | Language |
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dc.contributor.author | Santos, Ana Isabel | - |
dc.contributor.author | Lourenço, Ana Sofia | - |
dc.contributor.author | Simão, Sónia | - |
dc.contributor.author | Marques da Silva, Dorinda | - |
dc.contributor.author | Santos, Daniela Filipa | - |
dc.contributor.author | Onofre de Carvalho, Ana Paula | - |
dc.contributor.author | Pereira, Ana Catarina | - |
dc.contributor.author | Izquierdo-Álvarez, Alicia | - |
dc.contributor.author | Ramos, Elena | - |
dc.contributor.author | Morato, Esperanza | - |
dc.contributor.author | Marina, Anabel | - |
dc.contributor.author | Martínez-Ruiz, Antonio | - |
dc.contributor.author | Araújo, Inês Maria | - |
dc.date.accessioned | 2023-04-14T08:43:16Z | - |
dc.date.available | 2023-04-14T08:43:16Z | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 22132317 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/106654 | - |
dc.description.abstract | Nitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R-SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the proliferation of NSC. | pt |
dc.description.sponsorship | This work was supported by the COST action BM1005 (ENOG: European Network on Gasotransmitters), by the Spanish Government (grants PS09/00101, PI12/00875 and PI15/ 00107 from ISCIII and RTI2018-094203-B-I00 from AEI; co-financed by FEDER/ERDF) and by the Spanish-Portuguese Integrated Action grant PRI-AIBPT-2011-1015/E-10/12.The Proteomics Service of the CBMSO is a member of ProteoRed (PRB3-ISCIII), and is supported by grant PT13/0001/0024 of Spanish Government (cofinanced by FEDER/ ERDF). | pt |
dc.language.iso | eng | pt |
dc.publisher | Elsevier | pt |
dc.relation | SFRH/BD/77903/2011 | pt |
dc.relation | SFRH/BD/79308/2011 | pt |
dc.relation | PTDC/QUI-QFI/29319/2017 | pt |
dc.relation | PTDC/NEU-OSD/0473/2012 | pt |
dc.relation | UID/BIM/04773/2019 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt |
dc.subject | Nitric oxide S-nitrosylation | pt |
dc.subject | Neurogenesis | pt |
dc.subject | Neural stem cells | pt |
dc.subject | Neurogenesis Seizures | pt |
dc.subject | Hippocampus | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Cysteine | pt |
dc.subject.mesh | Mice | pt |
dc.subject.mesh | Nitric Oxide | pt |
dc.subject.mesh | Oxidation-Reduction | pt |
dc.subject.mesh | Protein Processing, Post-Translational | pt |
dc.subject.mesh | Proteomics | pt |
dc.subject.mesh | Sulfhydryl Compounds | pt |
dc.subject.mesh | Neural Stem Cells | pt |
dc.subject.mesh | S-Nitrosothiols | pt |
dc.title | Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics | pt |
dc.type | article | - |
degois.publication.firstPage | 101457 | pt |
degois.publication.title | Redox Biology | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1016/j.redox.2020.101457 | pt |
degois.publication.volume | 32 | pt |
dc.date.embargo | 2020-05-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.orcid | 0000-0003-2070-0285 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics.pdf | 2.04 MB | Adobe PDF | View/Open |
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