Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/107144
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Paula, Anabela | - |
dc.contributor.author | Laranjo, Mafalda | - |
dc.contributor.author | Marto, Carlos Miguel | - |
dc.contributor.author | Abrantes, Ana Margarida | - |
dc.contributor.author | Casalta-Lopes, João | - |
dc.contributor.author | Gonçalves, Ana Cristina | - |
dc.contributor.author | Ribeiro, Ana Bela Sarmento | - |
dc.contributor.author | Ferreira, Manuel M. | - |
dc.contributor.author | Botelho, Maria Filomena | - |
dc.contributor.author | Carrilho, Eunice | - |
dc.date.accessioned | 2023-06-12T08:17:02Z | - |
dc.date.available | 2023-06-12T08:17:02Z | - |
dc.date.issued | 2019-04-11 | - |
dc.identifier.issn | 1996-1944 | - |
dc.identifier.uri | https://hdl.handle.net/10316/107144 | - |
dc.description.abstract | (1) Background: When pulp exposure occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain the vitality and function of the tissue. The aim of this work was to assess the cytotoxicity and bioactivity of three different direct pulp capping materials, calcium hydroxide (Life®), mineral trioxide aggregate (WhiteProRoot®MTA) and calcium silicate (Biodentine™), in an odontoblast-like mouse cell line (MDPC-23). (2) Methods: Metabolic activity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (MTT)assay, viability by the sulforhodamine B (SRB) assay, and the type of death and cell cycle analysis by flow cytometry. Alkaline phosphatase was evaluated by polymerase chain reaction (PCR), and dentin sialoprotein expression was assessed by immunocytochemistry. Mineralization was determined by the Alizarin Red S colorimetric assay and quantified by spectrophotometry. (3) Results: Life® induced a decrease in metabolic activity and viability, which is associated with an increase cell death. WhiteProRoot®MTA and Biodentine™ induced similar effects in cytotoxicity assays, with an increase in the expression of dentin sialoprotein (DSP) and formation of mineralized deposits, especially with Biodentine™. (4) Conclusions: The results of WhiteProRoot®MTA confirm its indication for these therapies, justifying its recognition as the "gold standard". Biodentine™ may be an alternative, since they promote the same cellular response that mineral trioxide aggregate (MTA) does. | pt |
dc.description.sponsorship | GAI 2013 (Faculdade de Medicina da Universidade de Coimbra) | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | PEst-C/SAU/UI3282/2013 | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/NEU/04539/2013/PT | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | biocompatibility | pt |
dc.subject | biomaterials | pt |
dc.subject | cytotoxicity | pt |
dc.subject | dentinogenesis | pt |
dc.subject | odontoblast | pt |
dc.subject | pulp capping | pt |
dc.title | Biodentine™ Boosts, WhiteProRoot®MTA Increases and Life® Suppresses Odontoblast Activity | pt |
dc.type | article | pt |
degois.publication.firstPage | 1184 | pt |
degois.publication.issue | 7 | pt |
degois.publication.title | Materials | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/ma12071184 | - |
degois.publication.volume | 12 | pt |
dc.date.embargo | 2019-04-11 | * |
dc.identifier.pmid | 30978943 | - |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Strategic Project - UI 3282 - 2013-2014 | - |
crisitem.project.grantno | CNC. IBILI | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0003-0689-6007 | - |
crisitem.author.orcid | 0000-0001-9269-5417 | - |
crisitem.author.orcid | 0000-0003-4185-7871 | - |
crisitem.author.orcid | 0000-0003-1470-4802 | - |
crisitem.author.orcid | 0000-0002-4142-4841 | - |
crisitem.author.orcid | 0000-0002-5968-6161 | - |
crisitem.author.orcid | 0000-0001-7202-1650 | - |
crisitem.author.orcid | 0000-0002-5759-5557 | - |
Appears in Collections: | I&D IBILI - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Biodentine-boosts-WhiteProRootMTA-increases-and-Life-suppresses-odontoblast-activityMaterials.pdf | 2.6 MB | Adobe PDF | View/Open |
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