Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/109361
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sereno, José | - |
dc.contributor.author | Nunes, Sara | - |
dc.contributor.author | Rodrigues-Santos, Paulo | - |
dc.contributor.author | Vala, Helena | - |
dc.contributor.author | Rocha-Pereira, Petronila | - |
dc.contributor.author | Fernandes, João | - |
dc.contributor.author | Santos-Silva, Alice | - |
dc.contributor.author | Teixeira, Frederico | - |
dc.contributor.author | Reis, Flávio | - |
dc.date.accessioned | 2023-10-11T09:30:35Z | - |
dc.date.available | 2023-10-11T09:30:35Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 2314-6133 | pt |
dc.identifier.issn | 2314-6141 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/109361 | - |
dc.description.abstract | Protocols of conversion from cyclosporin A (CsA) to sirolimus (SRL) have been widely used in immunotherapy after transplantation to prevent CsA-induced nephropathy, but the molecular mechanisms underlying these protocols remain nuclear. This study aimed to identify the molecular pathways and putative biomarkers of CsA-to-SRL conversion in a rat model. Four animal groups (n = 6) were tested during 9 weeks: control, CsA, SRL, and conversion (CsA for 3 weeks followed by SRL for 6 weeks). Classical and emergent serum, urinary, and kidney tissue (gene and protein expression) markers were assessed. Renal lesions were analyzed in hematoxylin and eosin, periodic acid-Schiff, and Masson's trichrome stains. SRL-treated rats presented proteinuria and NGAL (serum and urinary) as the best markers of renal impairment. Short CsA treatment presented slight or even absent kidney lesions and TGF-β, NF- κβ, mTOR, PCNA, TP53, KIM-1, and CTGF as relevant gene and protein changes. Prolonged CsA exposure aggravated renal damage, without clear changes on the traditional markers, but with changes in serums TGF- β and IL-7, TBARs clearance, and kidney TGF-β and mTOR. Conversion to SRL prevented CsA-induced renal damage evolution (absent/mild grade lesions), while NGAL (serum versus urine) seems to be a feasible biomarker of CsA replacement to SRL. | pt |
dc.language.iso | eng | pt |
dc.publisher | Hindawi | pt |
dc.relation | SFRH/BD/63962/2009 | pt |
dc.relation | PEst-C/SAU/UI3282/2011 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Biomarkers | pt |
dc.subject.mesh | Cell Proliferation | pt |
dc.subject.mesh | Collagen | pt |
dc.subject.mesh | Cyclosporine | pt |
dc.subject.mesh | Gene Expression Regulation | pt |
dc.subject.mesh | Immunohistochemistry | pt |
dc.subject.mesh | Inflammation | pt |
dc.subject.mesh | Kidney | pt |
dc.subject.mesh | Kidney Diseases | pt |
dc.subject.mesh | Male | pt |
dc.subject.mesh | Neovascularization, Physiologic | pt |
dc.subject.mesh | Proteins | pt |
dc.subject.mesh | RNA, Messenger | pt |
dc.subject.mesh | Rats, Wistar | pt |
dc.subject.mesh | Sirolimus | pt |
dc.title | Conversion to sirolimus ameliorates cyclosporine-induced nephropathy in the rat: focus on serum, urine, gene, and protein renal expression biomarkers | pt |
dc.type | article | - |
degois.publication.firstPage | 576929 | pt |
degois.publication.lastPage | 17 | pt |
degois.publication.title | BioMed Research International | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1155/2014/576929 | pt |
degois.publication.volume | 2014 | pt |
dc.date.embargo | 2014-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Strategic Project - UI 3282 - 2011-2012 | - |
crisitem.author.researchunit | Centre for Earth and Space Research of the University of Coimbra | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-1663-3334 | - |
crisitem.author.orcid | 0000-0002-2601-0923 | - |
crisitem.author.orcid | 0000-0003-3401-9554 | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais I&D ICNAS - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais |
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