Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/109713
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Carvalho, Filipa | - |
dc.contributor.author | Duarte, João | - |
dc.contributor.author | Simoes, Ana Rita | - |
dc.contributor.author | Cruz, Pedro F. | - |
dc.contributor.author | Jones, John G. | - |
dc.date.accessioned | 2023-10-23T11:42:11Z | - |
dc.date.available | 2023-10-23T11:42:11Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 2314-6133 | pt |
dc.identifier.issn | 2314-6141 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/109713 | - |
dc.description.abstract | The (13)C-isotopomer enrichment of hepatic cytosolic acetyl-CoA of overnight-fed mice whose drinking water was supplemented with [U-(13)C]fructose, and [1-(13)C]glucose and p-amino benzoic acid (PABA) was quantified by (13)C NMR analysis of urinary N-acetyl-PABA. Four mice were given normal chow plus drinking water supplemented with 5% [1-(13)C]glucose, 2.5% [U-(13)C]fructose, and 2.5% fructose (Solution 1) overnight. Four were given chow and water containing 17.5% [1-(13)C]glucose, 8.75% [U-(13)C]fructose and 8.75% fructose (Solution 2). PABA (0.25%) was present in both studies. Urinary N-acetyl-PABA was analyzed by (13)C NMR. In addition to [2-(13)C]- and [1,2-(13)C]acetyl isotopomers from catabolism of [U-(13)C]fructose and [1-(13)C]glucose to acetyl-CoA, [1-(13)C]acetyl was also found indicating pyruvate recycling activity. This precluded precise estimates of [1-(13)C]glucose contribution to acetyl-CoA while that of [U-(13)C]fructose was unaffected. The fructose contribution to acetyl-CoA from Solutions 1 and 2 was 4.0 ± 0.4% and 10.6 ± 0.6%, respectively, indicating that it contributed to a minor fraction of lipogenic acetyl-CoA under these conditions. | pt |
dc.language.iso | eng | pt |
dc.publisher | Hindawi | pt |
dc.relation | PTDC/EEB-BIO/99810/2008 | pt |
dc.relation | PTDC/SAUMET/ 111398/2009 | pt |
dc.relation | REDE/1517/RMN/2005 | pt |
dc.relation | POCI/2010/FEDER | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Acetyl Coenzyme A | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Blood Glucose | pt |
dc.subject.mesh | Carbon Radioisotopes | pt |
dc.subject.mesh | Cytosol | pt |
dc.subject.mesh | Fatty Acids | pt |
dc.subject.mesh | Fructose | pt |
dc.subject.mesh | Glucose | pt |
dc.subject.mesh | Liver | pt |
dc.subject.mesh | Mice | pt |
dc.title | Noninvasive measurement of murine hepatic acetyl-CoA ¹³C-enrichment following overnight feeding with ¹³C-enriched fructose and glucose | pt |
dc.type | article | - |
degois.publication.firstPage | 638085 | pt |
degois.publication.lastPage | 7 | pt |
degois.publication.title | BioMed Research International | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1155/2013/638085 | pt |
degois.publication.volume | 2013 | pt |
dc.date.embargo | 2013-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Programa Operacional Competitividade e Internacionalização (COMPETE 2020) | - |
crisitem.author.orcid | 0000-0003-3578-0134 | - |
crisitem.author.orcid | 0000-0002-3745-3885 | - |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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