Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/110052
DC Field | Value | Language |
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dc.contributor.author | Azevedo, Ana Sofia | - |
dc.contributor.author | Grotek, Bartholomäus | - |
dc.contributor.author | Jacinto, António | - |
dc.contributor.author | Weidinger, Gilbert | - |
dc.contributor.author | Saúde, Leonor | - |
dc.date.accessioned | 2023-11-14T12:00:04Z | - |
dc.date.available | 2023-11-14T12:00:04Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1932-6203 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/110052 | - |
dc.description.abstract | Background: The zebrafish has the capacity to regenerate many tissues and organs. The caudal fin is one of the most convenient tissues to approach experimentally due to its accessibility, simple structure and fast regeneration. In this work we investigate how the regenerative capacity is affected by recurrent fin amputations and by experimental manipulations that block regeneration. Methodology/Principal Findings: We show that consecutive repeated amputations of zebrafish caudal fin do not reduce its regeneration capacity and do not compromise any of the successive regeneration steps: wound healing, blastema formation and regenerative outgrowth. Interfering with Wnt/ß-catenin signalling using heat-shock-mediated overexpression of Dickkopf1 completely blocks fin regeneration. Notably, if these fins were re-amputated at the non-inhibitory temperature, the regenerated caudal fin reached the original length, even after several rounds of consecutive Wnt/ß-catenin signalling inhibition and re-amputation. Conclusions/Significance: We show that the caudal fin has an almost unlimited capacity to regenerate. Even after inhibition of regeneration caused by the loss of Wnt/ß-catenin signalling, a new amputation resets the regeneration capacity within the caudal fin, suggesting that blastema formation does not depend on a pool of stem/progenitor cells that require Wnt/ßcatenin signalling for their survival. | pt |
dc.description.sponsorship | A.S.A. was supported by a (FCT) Fundac¸a˜o para a Cieˆncia e a Tecnologia (Portuguese Science and Technology Foundation) fellowship (SFRH/BD/33179/ 2007). L.S. was supported by two FCT grants PTDC/SAU-OBD/64628/2006 and PTDC/SAU-OBD/100202/2008. Work of the Weidinger lab was supported by the Deutsche Forschungsgemeinschaft (DFG) grant ‘‘Collaborative Research Center 655: Cells into Tissues: Stem Cell and Progenitor Commitment and Interactions during Tissue Formation," SFB655 to G.W. | pt |
dc.language.iso | eng | pt |
dc.publisher | Public Library of Science | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject.mesh | Animal Fins | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Extremities | pt |
dc.subject.mesh | Immunoenzyme Techniques | pt |
dc.subject.mesh | Intercellular Signaling Peptides and Proteins | pt |
dc.subject.mesh | RNA, Messenger | pt |
dc.subject.mesh | Regeneration | pt |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | pt |
dc.subject.mesh | Signal Transduction | pt |
dc.subject.mesh | Wnt Proteins | pt |
dc.subject.mesh | Wound Healing | pt |
dc.subject.mesh | Zebrafish | pt |
dc.subject.mesh | Zebrafish Proteins | pt |
dc.subject.mesh | beta Catenin | pt |
dc.subject.mesh | Amputation, Surgical | pt |
dc.title | The regenerative capacity of the zebrafish caudal fin is not affected by repeated amputations | pt |
dc.type | article | - |
degois.publication.firstPage | e22820 | pt |
degois.publication.issue | 7 | pt |
degois.publication.title | PLoS ONE | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1371/journal.pone.0022820 | pt |
degois.publication.volume | 6 | pt |
dc.date.embargo | 2011-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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The-regenerative-capacity-of-the-zebrafish-caudal-fin-is-not-affected-by-repeated-amputationsPLoS-ONE.pdf | 491.77 kB | Adobe PDF | View/Open |
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