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https://hdl.handle.net/10316/113565
Title: | Kazrin promotes dynein/dynactin-dependent traffic from early to recycling endosomes | Authors: | Hernandez-Perez, Ines Rubio, Javier Baumann, Adrian Girão, Henrique Ferrando, Miriam Rebollo, Elena Aragay, Anna M Geli, María Isabel |
Keywords: | cell biology; dynein/dynactin; endosome; kazrin; mouse; mouse embryonic fibroblast | Issue Date: | 25-Apr-2023 | Publisher: | eLife Sciences Publications | Project: | Agencia Estatal de Investigación BFU2017-82959-P Agencia Estatal de Investigación PID2020-120053GB-I00 Ministerio de Ciencia, Innovación y Universidades EQC2018-004541 EU FeDer Consejo Superior de Investigaciones Científicas CSIC1501/18 Ministerio de Ciencia, Innovación y Universidades BES-2015-071691 BES- 2012-053341 |
Serial title, monograph or event: | eLife | Volume: | 12 | Abstract: | Kazrin is a protein widely expressed in vertebrates whose depletion causes a myriad of developmental defects, in part derived from altered cell adhesion and migration, as well as failure to undergo epidermal to mesenchymal transition. However, the primary molecular role of kazrin, which might contribute to all these functions, has not been elucidated yet. We previously identified one of its isoforms, kazrin C, as a protein that potently inhibits clathrin-mediated endocytosis when overexpressed. We now generated kazrin knock-out mouse embryonic fibroblasts to investigate its endocytic function. We found that kazrin depletion delays juxtanuclear enrichment of internalized material, indicating a role in endocytic traffic from early to recycling endosomes. Consistently, we found that the C-terminal domain of kazrin C, predicted to be an intrinsically disordered region, directly interacts with several early endosome (EE) components, and that kazrin depletion impairs retrograde motility of these organelles. Further, we noticed that the N-terminus of kazrin C shares homology with dynein/dynactin adaptors and that it directly interacts with the dynactin complex and the dynein light intermediate chain 1. Altogether, the data indicate that one of the primary kazrin functions is to facilitate endocytic recycling by promoting dynein/dynactin-dependent transport of EEs or EE-derived transport intermediates to the recycling endosomes. | URI: | https://hdl.handle.net/10316/113565 | ISSN: | 2050-084X | DOI: | 10.7554/eLife.83793 | Rights: | openAccess |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais |
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Kazrin promotes dyneindynactin-dependent traffic from early to recycling endosomes.pdf | 11.71 MB | Adobe PDF | View/Open |
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