Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/113722
DC Field | Value | Language |
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dc.contributor.author | Sebastião, Ana Isabel | - |
dc.contributor.author | Mateus, Daniela | - |
dc.contributor.author | Carrascal, Mylène A. | - |
dc.contributor.author | Sousa, Cátia | - |
dc.contributor.author | Cortes, Luísa | - |
dc.contributor.author | Bachmann, Martin F | - |
dc.contributor.author | do Carmo, Anália | - |
dc.contributor.author | Matos, Ana M. | - |
dc.contributor.author | Sales, M. Goreti F. | - |
dc.contributor.author | Cruz, M. Teresa | - |
dc.date.accessioned | 2024-02-28T12:37:33Z | - |
dc.date.available | 2024-02-28T12:37:33Z | - |
dc.date.issued | 2023-03-02 | - |
dc.identifier.issn | 1999-4923 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/113722 | - |
dc.description.abstract | Dendritic cells (DCs) are the most specialized and proficient antigen-presenting cells. They bridge innate and adaptive immunity and display a powerful capacity to prime antigen-specific T cells. The interaction of DCs with the receptor-binding domain of the spike (S) protein from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a pivotal step to induce effective immunity against the S protein-based vaccination protocols, as well as the SARS-CoV-2 virus. Herein, we describe the cellular and molecular events triggered by virus-like particles (VLPs) containing the receptor-binding motif from the SARS-CoV-2 spike protein in human monocyte-derived dendritic cells, or, as controls, in the presence of the Toll-like receptors (TLR)3 and TLR7/8 agonists, comprehending the events of dendritic cell maturation and their crosstalk with T cells. The results demonstrated that VLPs boosted the expression of major histocompatibility complex molecules and co-stimulatory receptors of DCs, indicating their maturation. Furthermore, DCs' interaction with VLPs promoted the activation of the NF-kB pathway, a very important intracellular signalling pathway responsible for triggering the expression and secretion of proinflammatory cytokines. Additionally, co-culture of DCs with T cells triggered CD4+ (mainly CD4+Tbet+) and CD8+ T cell proliferation. Our results suggested that VLPs increase cellular immunity, involving DC maturation and T cell polarization towards a type 1 T cells profile. By providing deeper insight into the mechanisms of activation and regulation of the immune system by DCs, these findings will enable the design of effective vaccines against SARS-CoV-2. | pt |
dc.language.iso | eng | pt |
dc.publisher | MDPI | pt |
dc.relation | UIDB/04539/2020 | pt |
dc.relation | UIDP/04539/2020 | pt |
dc.relation | LA/P/0058/2020 | pt |
dc.relation | POCI-01-02B7- FEDER-069745 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | dendritic cells | pt |
dc.subject | SARS-CoV-2 | pt |
dc.subject | COVID-19 | pt |
dc.subject | memory T cell | pt |
dc.subject | adaptive immune response | pt |
dc.subject | virus-like particles | pt |
dc.title | CuMV VLPs Containing the RBM from SARS-CoV-2 Spike Protein Drive Dendritic Cell Activation and Th1 Polarization | pt |
dc.type | article | - |
degois.publication.firstPage | 825 | pt |
degois.publication.issue | 3 | pt |
degois.publication.title | Pharmaceutics | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/pharmaceutics15030825 | pt |
degois.publication.volume | 15 | pt |
dc.date.embargo | 2023-03-02 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - Associate Laboratory | - |
crisitem.author.researchunit | CIBB - Center for Innovative Biomedicine and Biotechnology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-1687-2968 | - |
crisitem.author.orcid | 0000-0002-7511-4358 | - |
crisitem.author.orcid | 0000-0001-5764-0023 | - |
crisitem.author.orcid | 0000-0001-9936-7336 | - |
crisitem.author.orcid | 0000-0001-9846-6754 | - |
Appears in Collections: | FCTUC Eng.Química - Artigos em Revistas Internacionais I&D CIBB - Artigos em Revistas Internacionais I&D CERES - Artigos em Revistas Internacionais FFUC- Artigos em Revistas Internacionais |
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CuMV-VLPs-Containing-the-RBM-from-SARSCoV2-Spike-Protein-Drive-Dendritic-Cell-Activation-and-Th1-PolarizationPharmaceutics.pdf | 2.78 MB | Adobe PDF | View/Open |
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