Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/114894
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Almeida, João F | - |
dc.contributor.author | Marques, Matilde S. | - |
dc.contributor.author | Oliveira, Vanessa | - |
dc.contributor.author | Egas, Conceição | - |
dc.contributor.author | Mil-Homens, Dalila | - |
dc.contributor.author | Viana, Romeu | - |
dc.contributor.author | Cleary, Daniel F. R. | - |
dc.contributor.author | Huang, Yusheng M | - |
dc.contributor.author | Fialho, Arsénio M | - |
dc.contributor.author | Teixeira, Miguel C | - |
dc.contributor.author | Gomes, Newton C. M. | - |
dc.contributor.author | Costa, Rodrigo | - |
dc.contributor.author | Keller-Costa, Tina | - |
dc.date.accessioned | 2024-04-16T13:56:04Z | - |
dc.date.available | 2024-04-16T13:56:04Z | - |
dc.date.issued | 2022-12-30 | - |
dc.identifier.issn | 1660-3397 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/114894 | - |
dc.description.abstract | Marine microbiomes are prolific sources of bioactive natural products of potential pharmaceutical value. This study inspected two culture collections comprising 919 host-associated marine bacteria belonging to 55 genera and several thus-far unclassified lineages to identify isolates with potentially rich secondary metabolism and antimicrobial activities. Seventy representative isolates had their genomes mined for secondary metabolite biosynthetic gene clusters (SM-BGCs) and were screened for antimicrobial activities against four pathogenic bacteria and five pathogenic Candida strains. In total, 466 SM-BGCs were identified, with antimicrobial peptide- and polyketide synthase-related SM-BGCs being frequently detected. Only 38 SM-BGCs had similarities greater than 70% to SM-BGCs encoding known compounds, highlighting the potential biosynthetic novelty encoded by these genomes. Cross-streak assays showed that 33 of the 70 genome-sequenced isolates were active against at least one Candida species, while 44 isolates showed activity against at least one bacterial pathogen. Taxon-specific differences in antimicrobial activity among isolates suggested distinct molecules involved in antagonism against bacterial versus Candida pathogens. The here reported culture collections and genome-sequenced isolates constitute a valuable resource of understudied marine bacteria displaying antimicrobial activities and potential for the biosynthesis of novel secondary metabolites, holding promise for a future sustainable production of marine drug leads. | pt |
dc.language.iso | eng | pt |
dc.relation | PTDC/BIA-MIC/6473/2014 | pt |
dc.relation | POCI-01-0145-FEDER-016531 | pt |
dc.relation | UIDB/04565/2020 | pt |
dc.relation | UIDP/04565/2020 | pt |
dc.relation | LA/P/0140/2020 | pt |
dc.relation | UIDP/50017/2020 | pt |
dc.relation | UIDB/50017/2020 | pt |
dc.relation | LA/P/0094/2020 | pt |
dc.relation | UIDB/04539/2020 | pt |
dc.relation | info:eu-repo/grantAgreement/UIDP/04539/2020 | pt |
dc.relation | LA/P/0058/2020 | pt |
dc.relation | POCI-01-0145-FEDER-022184 | pt |
dc.relation | CEECIND/00788/2017 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | bioprospection | pt |
dc.subject | biosynthetic gene clusters | pt |
dc.subject | blue biotechnology | pt |
dc.subject | culture collections | pt |
dc.subject | genomics | pt |
dc.subject | marine bacteria | pt |
dc.subject.mesh | Animals | pt |
dc.subject.mesh | Humans | pt |
dc.subject.mesh | Secondary Metabolism | pt |
dc.subject.mesh | Bacteria | pt |
dc.subject.mesh | Multigene Family | pt |
dc.subject.mesh | Candida | pt |
dc.subject.mesh | Phylogeny | pt |
dc.subject.mesh | Porifera | pt |
dc.subject.mesh | Anti-Infective Agents | pt |
dc.subject.mesh | Anthozoa | pt |
dc.title | Marine Sponge and Octocoral-Associated Bacteria Show Versatile Secondary Metabolite Biosynthesis Potential and Antimicrobial Activities against Human Pathogens | pt |
dc.type | article | - |
degois.publication.firstPage | 34 | pt |
degois.publication.issue | 1 | pt |
degois.publication.title | Marine Drugs | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/md21010034 | pt |
degois.publication.volume | 21 | pt |
dc.date.embargo | 2022-12-30 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.project.grantno | Institute for Bioengineering and Biosciences -iBB | - |
crisitem.project.grantno | Institute for Bioengineering and Biosciences | - |
crisitem.project.grantno | Centre for Environmental and Marine Studies | - |
crisitem.project.grantno | Centre for Environmental and Marine Studies - CESAM | - |
crisitem.project.grantno | Centre for Environmental and Marine Studies | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - CIBB | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology | - |
crisitem.project.grantno | Center for Innovative Biomedicine and Biotechnology - Associate Laboratory | - |
crisitem.author.orcid | 0000-0002-2307-5414 | - |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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