Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/114916
Title: | Circulating microRNA levels differ in the early stages of insulin resistance in prepubertal children with obesity | Authors: | Santos, Diana Porter-Gill, Patricia Goode, Grace Delhey, Leanna Sørensen, Anja Elaine Rose, Shannon Børsheim, Elisabet Dalgaard, Louise Torp Carvalho, Eugenia |
Keywords: | Prepubertal obesity; Insulin resistance; microRNAs; Early molecular alterations | Issue Date: | 1-Jan-2023 | Publisher: | Elsevier | Project: | CENTRO-01-0145-FEDER-000012 POCI-01-0145-FEDER-007440 UIDB/04539/2020 info:eu-repo/grantAgreement/UIDP/04539/2020 SRFH/BD/144199/2019 NNF17SA0031406 |
Serial title, monograph or event: | Life Sciences | Volume: | 312 | Abstract: | Aims: The increasing prevalence of childhood obesity escalates the risk for related complications. Circulating microRNAs (miRNAs) have been suggested as good predictive markers of insulin resistance in those with obesity. The aim was to identify a circulating miRNA profile that reflects insulin resistance in prepubertal children with obesity. Material and methods: Plasma miRNAs were measured in prepubertal children (n = 63, 5–9 years) using TaqMan Advanced miRNA Human Serum/Plasma plates and then were validated by RT-qPCR. Subjects were divided into normal weight (n = 20, NW) and overweight or obese (n = 43, OW/OB) groups according to their BMI z-scores. The OW/OB group was further subdivided into insulin sensitive or metabolically healthy obese (n = 26, MHO) and insulin resistant or metabolically unhealthy obese (n = 17, MUO) according to HOMA-IR. Key findings: While no differences were observed in the fasting plasma glucose levels, serum insulin levels were significantly elevated in the OW/OB compared to the NW group. Of 188 screened miRNAs, eleven were differentially expressed between the NW and OW/OB groups. Validation confirmed increased circulating levels of miR-146a-5p and miR-18a-5p in the OW/OB group, which correlated with BMI z-score. Interestingly, miR- 146a-5p was also correlated with HOMA-IR index. While only miR-18a-5p was upregulated in the OW/OB children, independently of their degree of insulin sensitivity, miR-146-5p, miR-423-3p and miR-152-3p were associated with insulin resistance. Significance: The present study provides evidence of molecular alterations that occur early in life in prepubertal obesity. These alterations may potentially be crucial for targeted prevention or prompt precision therapeutic development and subsequent interventions. | URI: | https://hdl.handle.net/10316/114916 | ISSN: | 00243205 | DOI: | 10.1016/j.lfs.2022.121246 | Rights: | openAccess |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Circulating microRNA levels differ in the early stages of insulin resistance in prepubertal children with obesity.pdf | 821.76 kB | Adobe PDF | View/Open |
Page view(s)
129
checked on Oct 2, 2024
Download(s)
126
checked on Oct 2, 2024
Google ScholarTM
Check
Altmetric
Altmetric
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.