Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/12769
DC Field | Value | Language |
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dc.contributor.author | Fonseca, Carlos P. | - |
dc.contributor.author | Montezinho, Liliana P. | - |
dc.contributor.author | Baltazar, Graça | - |
dc.contributor.author | Layden, Brian | - |
dc.contributor.author | Freitas, Duarte M. | - |
dc.contributor.author | Geraldes, Carlos F. G. C. | - |
dc.contributor.author | Castro, M. Margarida C. A. | - |
dc.date.accessioned | 2010-03-09T10:49:15Z | - |
dc.date.available | 2010-03-09T10:49:15Z | - |
dc.date.issued | 2000 | - |
dc.identifier.citation | Metal-Based Drugs. 7:6 (2000) 357-364 | en_US |
dc.identifier.issn | 0793-0291 | - |
dc.identifier.uri | https://hdl.handle.net/10316/12769 | - |
dc.description.abstract | Li(+) influx by bovine chromaffin cells, obtained from bovine adrenal medulla, was studied in intact cell suspensions using (7)Li NMR spectroscopy with the shift reagent [Tm(HDOTP)](4-). The influx rate constants, k(i), were determined in the absence and in the presence of two Na(+) membrane transport inhibitors. The values obtained indicate that both voltage sensitive Na(+) channels and (Na(+)/K(+))-ATPase play an important role in Li(+) uptake by these cells. (7)Li NMR T(1) and T(2) relaxation times for intracellular Li(+) in bovine chromaffin cells provided a T(1)/T(2) ratio of 305, showing that Li(+) is highly, immobilized due to strong binding to intracellular structures. Using fluorescence spectroscopy and the Mg(2+) fluorescent probe, furaptra, the free intracellular Mg(2+) concentration in the bovine chromaffin cells incubated with 15 mM LiCl was found to increase by about mM after the intracellular Li(+) concentration reached a steady state. Therefore, once inside the cell, Li(+) is able to displace Mg(2+) from its binding sites | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Hindawi Publishing Corporation | en_US |
dc.rights | openAccess | en_US |
dc.title | Li influx and binding, and li/mg competition in bovine chromaffin cell suspensions as studied by li NMR and fluorescence spectroscopy | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1155/MBD.2000.357 | - |
uc.controloAutoridade | Sim | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0002-0837-8329 | - |
crisitem.author.orcid | 0000-0001-6811-3878 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Competition in Bovine Chromaffin Cell Suspensions as Studied.pdf | 1.16 MB | Adobe PDF | View/Open |
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