Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/12770
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Montezinho, Liliana P. | - |
dc.contributor.author | Fonseca, Carla P. | - |
dc.contributor.author | Geraldes, Carlos F. G. C. | - |
dc.contributor.author | Castro, M. Margarida C. A. | - |
dc.date.accessioned | 2010-03-09T10:55:28Z | - |
dc.date.available | 2010-03-09T10:55:28Z | - |
dc.date.issued | 2002 | - |
dc.identifier.citation | Metal-Based Drugs. 9:1-2 (2002) 69-80 | en_US |
dc.identifier.issn | 0793-0291 | - |
dc.identifier.uri | https://hdl.handle.net/10316/12770 | - |
dc.description.abstract | Magnesium is an essential element for all living systems. The quantification of free intracellular Mg(2+) concentration ([Mg(2+)](i)) is of utmost importance since changes in its basal value may be an indication of different pathologies due to abnormalities of Mg(2+) metabolism. In this work we used (31)P NMR and fluorescence spectroscopy to determine the resting [Mg(2+)](i) in bovine chromaffin cells, a neuron-like cellular model, as well as confocal laser scanning microscopy to study the free Mg(2+) spatial distribution in these cells. (31)P NMR spectroscopy did not prove to be effective for the determination of [Mg(2+)](i) in this particular case due to some special morphological and physiological properties of this cell type. A basal [Mg(2+)](i) value of 0.551 +/- 0.008 mM was found for these cells using fluorescence spectroscopy and the Mg(2+)-sensitive probe furaptra; this value falls in the concentration range reported in the literature for neurons from different sources. This technique proved to be an accurate and sensitive tool to determine the [Mg(2+)](i).lntraceilular free Mg(2+) seems to be essentially localized in the nucleus and around it, as shown by confocal microscopy with the Mg(2+)-sensitive probe Magnesium Green. It was not possible to derive any conclusion about free Mg(2+) localization inside the chromaffin granules and/or in the cytoplasm due to the lack of sufficient spatial resolution and to probe compartmentalization | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Hindawi Publishing Corporation | en_US |
dc.rights | openAccess | en_US |
dc.title | Quantification and Localization of Intracellular Free Mg2+ in Bovine Chromaffin Cells | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1155/MBD.2002.69 | - |
uc.controloAutoridade | Sim | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0002-0837-8329 | - |
crisitem.author.orcid | 0000-0001-6811-3878 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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Quantification and Localization of Intracellular Free.pdf | 1.48 MB | Adobe PDF | View/Open |
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