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https://hdl.handle.net/10316/44487
Title: | Intracellular water - an overlooked drug target? Cisplatin impact in cancer cells probed by neutrons | Authors: | Marques, M. P. M. Carvalho, A. L. M. Batista de Sakai, V Garcia Hatter, L Carvalho, L. A. E. Batista de |
Issue Date: | 25-Jan-2017 | Serial title, monograph or event: | Physical chemistry chemical physics : PCCP | Volume: | 19 | Issue: | 4 | Abstract: | The first neutron scattering study on human nucleated cells is reported, addressing the subject of solvent-slaving to a drug by probing intracellular water upon drug exposure. Inelastic and quasi-elastic neutron scattering spectroscopy with isotope labelling was applied for monitoring interfacial water response to the anticancer drug cisplatin, in the low prognosis human metastatic breast cancer cells MDA-MB-231. Optical vibrational data were also obtained for lyophilised cells. Concentration-dependent dynamical changes evidencing a progressive mobility reduction were unveiled between untreated and cisplatin-exposed samples, concurrent with variations in the native organisation of water molecules within the intracellular medium as a consequence of drug action. The results thus obtained yielded a clear picture of the intracellular water response to cisplatin and constitute the first reported experimental proof of a drug impact on the cytomatrix by neutron techniques. This is an innovative way of tackling a drug's pharmacodynamics, searching for alternative targets of drug action. | URI: | https://hdl.handle.net/10316/44487 | DOI: | 10.1039/c6cp05198g | Rights: | embargoedAccess |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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PCCP_17.pdf | 3.77 MB | Adobe PDF | View/Open |
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