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https://hdl.handle.net/10316/4679
Title: | GABA release by basket cells onto Purkinje cells, in rat cerebellar slices, is directly controlled by presynaptic purinergic receptors, modulating Ca2+ influx | Authors: | Donato, Roberta Rodrigues, Ricardo J. Takahashi, Michiko Tsai, Ming Chi Soto, David Miyagi, Kana Villafuertes, Rosa Gomez Cunha, Rodrigo A. Edwards, Frances A. |
Keywords: | Purkinje cell; Cerebellar cortex; Purinergic; GABA release; Calcium influx; Calcium stores; Synaptic transmission; Presynaptic receptors | Issue Date: | 1-Sep-2008 | Citation: | Cell Calcium. In Press, Corrected Proof: | Abstract: | In many brain regions, Ca2+ influx through presynaptic P2X receptors influences GABA release from interneurones. In patch-clamp recordings of Purkinje cells (PCs) in rat cerebellar slices, broad spectrum P2 receptor antagonists, PPADS (30 [mu]M) or suramin (12 [mu]M), result in a decreased amplitude and increased failure rate of minimal evoked GABAergic synaptic currents from basket cells. The effect is mimicked by desensitizing P2X1/3-containing receptors with [alpha],[beta]-methylene ATP. This suggests presynaptic facilitation of GABA release via P2XR-mediated Ca2+ influx activated by endogenously released ATP. In contrast, activation of P2Y4 receptors (using UTP, 30 [mu]M, but not P2Y1 or P2Y6 receptor ligands) results in inhibition of GABA release. Immunological studies reveal the presence of most known P2Rs in >=20% of GABAergic terminals in the cerebellum. P2X3 receptors and P2Y4 receptors occur in approximately 60% and 50% of GABAergic synaptosomes respectively and are localized presynaptically. Previous studies report that PC output is also influenced by postsynaptic purinergic receptors located on both PCs and interneurones. | URI: | https://hdl.handle.net/10316/4679 | DOI: | 10.1016/j.ceca.2008.03.006 | Rights: | openAccess |
Appears in Collections: | FMUC Medicina - Artigos em Revistas Internacionais |
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