Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/5802
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Almeida, L. Pereira de | - |
dc.contributor.author | Zala, D. | - |
dc.contributor.author | Aebischer, P. | - |
dc.contributor.author | Déglon, N. | - |
dc.date.accessioned | 2008-09-26T17:42:01Z | - |
dc.date.available | 2008-09-26T17:42:01Z | - |
dc.date.issued | 2001 | en_US |
dc.identifier.citation | Neurobiology of Disease. 8:3 (2001) 433-446 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/5802 | - |
dc.description.abstract | Neurodegenerative diseases represent promising targets for gene therapy approaches provided effective transfer vectors. In the present study, we evaluated the effectiveness of LacZ-expressing lentiviral vectors with two different internal promoters, the mouse phosphoglycerate kinase 1 (PGK) and cytomegalovirus (CMV), to infect striatal cells. The intrastriatal injection of lenti-[beta]-Gal vectors lead to 207, 400 ± 11,500 and 303,100 ± 4,300 infected cells in adult rats, respectively. Importantly, the [beta]-galactosidase activity was higher in striatal extracts from PGK-LacZ-injected animals as compared to CMV-LacZ animals. The efficacy of the system was further examined with a potential therapeutic gene for the treatment of Huntington's disease, the human ciliary neurotrophic factor (CNTF). PGK-LacZ- or PGK-CNTF-expressing viruses were stereotaxically injected into the striatum of rats, 3 weeks later the animals were unilaterally lesioned with 180 nmol of quinolinic acid (QA). Control animals displayed 148 ± 43 apomorphine-induced rotations ipsilateral to the lesion 5 days postlesion as compared to 26 ± 22 turns/45 min in the CNTF-treated group. The extent of the striatal damage was significantly diminished in the CNTF-treated rats as indicated by the 52 ± 9.7% decrease of the lesion volume and the sparing of DARPP-32, ChAT and NADPH-d neuronal populations. These results further establish that lentiviruses may represent an efficient gene delivery system for the screening of therapeutic molecules in Huntington's disease. | en_US |
dc.description.uri | http://www.sciencedirect.com/science/article/B6WNK-459HT27-6/1/34ed72189337f85bad52e94464dadd04 | en_US |
dc.format.mimetype | aplication/PDF | en |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.subject | Huntington's disease; lentiviral vector; gene therapy; ciliary neurotrophic factor; quinolinic acid lesion model | en_US |
dc.title | Neuroprotective Effect of a CNTF-Expressing Lentiviral Vector in the Quinolinic Acid Rat Model of Huntington's Disease | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1006/nbdi.2001.0388 | - |
item.openairetype | article | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CIBB - Center for Innovative Biomedicine and Biotechnology | - |
crisitem.author.orcid | 0000-0001-5831-3307 | - |
crisitem.author.orcid | 0000-0002-0052-8011 | - |
Appears in Collections: | FFUC- Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
file9fc9d68c420541a7a2c2ed06151beea4.pdf | 629.02 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.