Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/80892
DC Field | Value | Language |
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dc.contributor.author | Martins, André F | - |
dc.contributor.author | Prata, M. I. M. | - |
dc.contributor.author | Rodrigues, S. P. J. | - |
dc.contributor.author | Geraldes, Carlos F. G. C. | - |
dc.contributor.author | Riss, P. J. | - |
dc.contributor.author | Amor-Coarasa, A. | - |
dc.contributor.author | Burchardt, C. | - |
dc.contributor.author | Kroll, C. | - |
dc.contributor.author | Roesch, F. | - |
dc.date.accessioned | 2018-09-25T10:36:52Z | - |
dc.date.available | 2018-09-25T10:36:52Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1555-4317 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/80892 | - |
dc.description.abstract | Recent reports have claimed a superior performance of HEPES buffer in comparison to alternative buffer systems for (67/68) Ga labeling in aqueous media. In this paper we report spectroscopic ((1) H and (71) Ga NMR), radiochemical, mass spectrometry and theoretical modeling studies on the Ga(3+)/HEPES system (HEPES = N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid) performed with the aim of elucidating a potential contribution of HEPES in the (68/67) Ga radiolabeling process. Our results demonstrate that HEPES acts as a weakly but competitive chelator of Ga(3+) and that this interaction depends on the relative Ga(3+): HEPES concentration. A by-product formed in the labeling mixture has been identified as a [(68) Ga]Ga(HEPES) complex via chromatographic comparison with the nonradioactive analog. The formation of this complex was verified to compete with [(68) Ga]Ga(NOTA) complexation at low NOTA concentration. Putative chelation of Ga(3+) by the hydroxyl and adjacent ring nitrogen of HEPES is proposed on the basis of (1)H NMR shifts induced by Ga(3+) and theoretical modeling studies. | pt |
dc.language.iso | por | pt |
dc.rights | openAccess | pt |
dc.title | Spectroscopic, radiochemical, and theoretical studies of the Ga3+-N-2-hydroxyethyl piperazine-N'-2-ethanesulfonic acid (HEPES buffer) system: evidence for the formation of Ga3+ - HEPES complexes in (68) Ga labeling reactions | pt |
dc.type | article | - |
degois.publication.firstPage | 265-73 | pt |
degois.publication.lastPage | 273 | pt |
degois.publication.issue | 3 | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1002/cmmi.1517 | pt |
degois.publication.volume | 8 | pt |
dc.date.embargo | 2013-01-01 | * |
dc.date.periodoembargo | 0 | pt |
dc.identifier.pmid | 23606430 | - |
uc.controloAutoridade | Sim | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | pt | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0003-2888-0073 | - |
crisitem.author.orcid | 0000-0002-4640-7039 | - |
crisitem.author.orcid | 0000-0002-0837-8329 | - |
Appears in Collections: | FCTUC Química - Artigos em Revistas Internacionais |
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File | Description | Size | Format | |
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Martins_et_al-2013-Contrast_Media_%26_Molecular_Imaging.pdf | 399.68 kB | Adobe PDF | View/Open |
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