Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/8320
DC Field | Value | Language |
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dc.contributor.author | Pereira, Daniela B. | - |
dc.contributor.author | Rebola, Nelson | - |
dc.contributor.author | Rodrigues, Ricardo J. | - |
dc.contributor.author | Cunha, Rodrigo A. | - |
dc.contributor.author | Carvalho, Arsélio P. | - |
dc.contributor.author | Duarte, Carlos B. | - |
dc.date.accessioned | 2009-02-09T14:33:18Z | - |
dc.date.available | 2009-02-09T14:33:18Z | - |
dc.date.issued | 2006 | en_US |
dc.identifier.citation | Journal of Neuroscience Research. 83:5 (2006) 832-844 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/8320 | - |
dc.description.abstract | Brain-derived neurotrophic factor (BDNF) modulates glutamatergic excitatory transmission in hippocampal primary cultures by acting at a presynaptic locus. Although it has been suggested that BDNF also modulates adult hippocampus glutamatergic transmission, this remains a matter of controversy. To clarify a putative role for this neurotrophin in the modulation of glutamate release we applied exogenous BDNF to isolated adult rat hippocampal nerve terminals. BDNF, at 100 ng/ml, potentiated by 25% the K+-evoked release of [3H]glutamate from hippocampal synaptosomes. The small effect of BDNF on [3H]glutamate release correlated with a modest increase in phospholipase Cgamma (PLCgamma) phosphorylation, and with the lack of effect of BDNF on extracellular-signal regulated kinase (ERK) and Akt phosphorylation. Immunocytochemistry studies demonstrated that only about one-third of glutamatergic synaptosomes were positive for TrkB immunoreactivity. Furthermore, biotinylation and subsynaptic fractionation studies showed that only one-fourth of total full-length TrkB was present at the plasma membrane, evenly distributed between the presynaptic active zone and the postsynaptic density. These results indicate that BDNF modulates synaptic transmission presynaptically in a small subset of hippocampal glutamatergic synapses that contain TrkB and that express the receptor on the plasma membrane. © 2006 Wiley-Liss, Inc. | en_US |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.title | Trkb receptors modulation of glutamate release is limited to a subset of nerve terminals in the adult rat hippocampus | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1002/jnr.20784 | en_US |
uc.controloAutoridade | Sim | - |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0002-7631-743X | - |
crisitem.author.orcid | 0000-0003-2550-6422 | - |
crisitem.author.orcid | 0000-0002-1474-0208 | - |
Appears in Collections: | FCTUC Ciências da Vida - Artigos em Revistas Internacionais |
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