Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/8514
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Halldner, Linda | - |
dc.contributor.author | Lopes, Luisa V. | - |
dc.contributor.author | Daré, Elisabetta | - |
dc.contributor.author | Lindström, Karin | - |
dc.contributor.author | Johansson, Björn | - |
dc.contributor.author | Ledent, Catherine | - |
dc.contributor.author | Cunha, Rodrigo A. | - |
dc.contributor.author | Fredholm, Bertil B. | - |
dc.date.accessioned | 2009-02-17T10:51:18Z | - |
dc.date.available | 2009-02-17T10:51:18Z | - |
dc.date.issued | 2004 | en_US |
dc.identifier.citation | Naunyn-Schmiedeberg's Archives of Pharmacology. 370:4 (2004) 270-278 | en_US |
dc.identifier.uri | https://hdl.handle.net/10316/8514 | - |
dc.description.abstract | The adenosine receptor agonist 2-[ p-(2-carboxyethyl)phenylethylamino]-5'- N-ethylcarboxamidoadenosine (CGS 21680) is generally considered to be a selective adenosine A 2A receptor ligand. However, the compound has previously been shown to exhibit binding characteristics that are not compatible with adenosine A 2A receptor binding, at least in brain regions other than the striatum. We have examined binding of [ 3H]CGS 21680 and of antagonist radioligands with high selectivity for adenosine A 1 or A 2A receptors to hippocampus and striatum of mice lacking either adenosine A 1 (A1R (-/-)) or A 2A (A2AR (-/-)) receptors. Both receptor autoradiography and membrane binding techniques were used for this purpose and gave similar results. There were no significant changes in the binding of the A 1 receptor antagonist [ 3H]DPCPX in mice lacking A 2A receptors, or in the binding of the A 2A receptor antagonists [ 3H]SCH 58261 and [ 3H]ZM 241385 in mice lacking A 1 receptors. Furthermore, [ 3H]CGS 21680 binding in striatum was abolished in the A2AR (-/-), and essentially unaffected in striatum from mice lacking A 1 receptors. In hippocampus, however, binding of [ 3H]CGS 21680 remained in the A2AR (-/-), whereas binding was virtually abolished in the A1R (-/-). There were no adaptive alterations in A 2A receptor expression in this region in A1R (-/-) mice. Thus, most of the [ 3H]CGS 21680 binding in hippocampus is dependent on the presence of adenosine A 1 receptors, but not on A 2A receptors, indicating a novel binding site or novel binding mode. | en_US |
dc.language.iso | eng | eng |
dc.rights | openAccess | eng |
dc.title | Binding of adenosine receptor ligands to brain of adenosine receptor knock-out mice: evidence that CGS 21680 binds to A 1 receptors in hippocampus | en_US |
dc.type | article | en_US |
dc.identifier.doi | 10.1007/s00210-004-0970-1 | en_US |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CNC - Center for Neuroscience and Cell Biology | - |
crisitem.author.orcid | 0000-0003-2550-6422 | - |
Aparece nas coleções: | FMUC Medicina - Artigos em Revistas Internacionais |
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