Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/88171
DC Field | Value | Language |
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dc.contributor.author | Josephine, Blersch | - |
dc.contributor.author | Vitor, Francisco | - |
dc.contributor.author | Rebelo, Catarina | - |
dc.contributor.author | Adrian, Jimenez-Balsa | - |
dc.contributor.author | Antunes, Helena | - |
dc.contributor.author | Carlo, Gonzato | - |
dc.contributor.author | Pinto, Sandra | - |
dc.contributor.author | Simões, Susana | - |
dc.contributor.author | Klaus, Liedl | - |
dc.contributor.author | Karsten, Haupt | - |
dc.contributor.author | Ferreira, Lino | - |
dc.date.accessioned | 2019-11-26T11:15:20Z | - |
dc.date.available | 2019-11-26T11:15:20Z | - |
dc.date.issued | 2019-11-14 | - |
dc.identifier.uri | https://hdl.handle.net/10316/88171 | - |
dc.description.abstract | RNA-based therapies offer a wide range of therapeutic interventions including for the treatment of skin diseases; however, the strategies to deliver efficiently these biomolecules are still limited due to obstacles related to the cellular uptake and cytoplasmic delivery. Herein, we synthesized a triggerable polymeric nanoparticle (NP) library composed by 160 formulations, presenting physico-chemical diversity and differential responsiveness to light. Six formulations were more efficient (up to 500%) than commercial Lipofectamine in gene knockdown activity. These formulations had differential internalization by skin cells and the endosomal escape was rapid (minutes range) as shown by the recruitment of galectin-8. The NPs were effective in the release of siRNA and miRNA. Acute skin wounds treated with the top hit NP complexed with miRNA-150-5p healed faster than wounds treated with scramble miRNA. Light-activatable NPs offer a new strategy to deliver topically non-coding RNAs. | pt |
dc.description.sponsorship | ERA Chair project (ERA@UC, ref:669088) through EU Horizon 2020 program, the POCI-01-0145-FEDER-016390 (acronym: CANCEL STEM) and POCI-01- 0145-FEDER-029414 (acronym: LIghtBRARY) projects through Compete 2020 and FCT programs. NMR data was collected at the UC-NMR facility which is supported in part by FEDER – European Regional Development Fund through the COMPETE Programme (Operational Programme for Competitiveness) and by National Funds through FCT – Fundação para a Ciência e a Tecnologia (Portuguese Foundation for Science and Technology) through grants REEQ/481/QUI/2006, RECI/QEQ-QFI/0168/2012, CENTRO-07-CT62- FEDER-002012, and Rede Nacional de Ressonância Magnética Nuclear (RNRMN). | - |
dc.language.iso | eng | pt |
dc.publisher | Wiley | pt |
dc.rights | openAccess | pt |
dc.title | A light-triggerable nanoparticle library for the controlled release of non-coding RNAs | pt |
dc.type | article | - |
degois.publication.title | Angewandte Chemie International Edition | pt |
dc.relation.publisherversion | https://onlinelibrary.wiley.com/doi/10.1002/anie.201911398 | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.1002/anie.201911398 | pt |
dc.date.embargo | 2019-11-14 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
Appears in Collections: | I&D CNC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Manuscript.pdf | 1.68 MB | Adobe PDF | View/Open | |
Supporting Information.pdf | 4.72 MB | Adobe PDF | View/Open |
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