Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/90918
DC Field | Value | Language |
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dc.contributor.author | Sato, Tabata | - |
dc.contributor.author | Mello, Daphne | - |
dc.contributor.author | Vasconcellos, Luana | - |
dc.contributor.author | Valente, Artur J. M. | - |
dc.contributor.author | Borges, Alexandre Walmott | - |
dc.date.accessioned | 2020-09-10T14:50:13Z | - |
dc.date.available | 2020-09-10T14:50:13Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 1422-0067 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/90918 | - |
dc.description.abstract | Chitosan-DNA (CS-DNA) and Chitosan-Pectin (CS-P) hydrogels were formulated as a sustained drug delivery carrier for drug delivery. For this, hydrogels were prepared by emulsion technique: mixing aqueous phase of the CS and DNA or P solution with benzyl alcohol using a high-performance dispersing instrument. Green Propolis (GP) was incorporated by imbibition: hydrogels were placed in GP aqueous solution (70 µg/mL) for 2 h. The specimens were freeze-dried and then characterized using different techniques. In vitro cell viability and morphology were also performed using the MG63 cell line. The presence of P was evidenced by the occurrence of a strong band at 1745 cm-1, also occurring in the blend. DNA and CS-DNA showed a strong band at 1650 cm-1, slightly shifted from the chitosan band. The sorption of GP induced a significant modification of the gel surface morphology and some phase separation occurs between chitosan and DNA. Drug release kinetics in water and in saliva follow a two-step mechanism. Significant biocompatibility revealed that these hydrogels were non-toxic and provided acceptable support for cell survival. Thus, the hydrogel complexation of chitosan with DNA and with Pectin provides favorable micro-environment for cell growth and is a viable alternative drug delivery system for Green Propolis. | pt |
dc.language.iso | eng | pt |
dc.relation | UIDP/QUI/UI0313/2020 | pt |
dc.relation | UIDB/QUI/0313/2020 | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.subject | DNA | pt |
dc.subject | Chitosan | pt |
dc.subject | Hydrogel | pt |
dc.subject | Pectin | pt |
dc.subject | Propolis | pt |
dc.title | Chitosan-Based Coacervate Polymers for Propolis Encapsulation: Release and Cytotoxicity Studies | pt |
dc.type | article | - |
degois.publication.firstPage | 4561 | pt |
degois.publication.issue | 12 | pt |
degois.publication.title | International Journal of Molecular Science | pt |
dc.relation.publisherversion | https://www.mdpi.com/1422-0067/21/12/4561 | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.3390/ijms21124561 | pt |
degois.publication.volume | 21 | pt |
dc.date.embargo | 2020-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.researchunit | CQC - Coimbra Chemistry Centre | - |
crisitem.author.parentresearchunit | Faculty of Sciences and Technology | - |
crisitem.author.orcid | 0000-0002-4612-7686 | - |
Appears in Collections: | I&D CQC - Artigos em Revistas Internacionais |
Files in This Item:
File | Description | Size | Format | |
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ijms-21-04561-v2.pdf | 3.61 MB | Adobe PDF | View/Open |
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