Please use this identifier to cite or link to this item:
https://hdl.handle.net/10316/96386
DC Field | Value | Language |
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dc.contributor.author | Proença, P. | - |
dc.contributor.author | Vidinha, J. | - |
dc.contributor.author | Teixeira, H. | - |
dc.contributor.author | Castanheira, F. | - |
dc.contributor.author | Mendonça, M. C. | - |
dc.contributor.author | Sousa, E. | - |
dc.contributor.author | Mustra, C. | - |
dc.contributor.author | Franco, J. M | - |
dc.contributor.author | Corte-Real, F. | - |
dc.contributor.author | Vieira, D. N. | - |
dc.date.accessioned | 2021-11-16T15:02:58Z | - |
dc.date.available | 2021-11-16T15:02:58Z | - |
dc.date.issued | 2010 | - |
dc.identifier.isbn | 9789892601731 | pt |
dc.identifier.uri | https://hdl.handle.net/10316/96386 | - |
dc.description.abstract | Paraquat (PQ) is a toxic quaternary ammonium compound widely used in agriculture. Numerous cases of paraquat intoxication have been reported either accidentally or intentionally as suicidal attempts. A method for the determination of the herbicide PQ in blood and urine samples was developed using liquid chromatography-(electrospray ionization) mass spectrometry (LCESI-MS), following extraction with Oasis® WCX solid-phase cartridges. Chromatographic separation was achieved using an Atlantis® HILIC silica column, eluted isocratically with acetonitrile and ammonium formate (200mM) buffer, pH 3.8, at a 300 µL/min flow rate. Quantitation was achieved by the addition of ethyl paraquat as internal standard (IS). The compounds were detected monitoring two ions for PQ (m/z 185 and m/z 171) and m/z 213 for the IS. The method was applied to determine PQ in two cases: a non fatal case, a 42-year-old female with 0.13 µg/mL PQ concentration in blood and 6.29 µg/mL in urine and a lethal case, a 51-year-old male with 0.27 µg/ mL PQ concentration in blood. The authors developed a specific, sensitive and rapid assay for the identification and quantification of PQ, very important for monitoring suspected paraquat intoxications in hospitals and subsequently help in the treatment of these patients. | pt |
dc.language.iso | eng | pt |
dc.publisher | Imprensa da Universidade de Coimbra; International Academy of Legal Medicine | pt |
dc.rights | openAccess | pt |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt |
dc.title | Determination of paraquat in blood and urine by liquid chromatography-electrospray-mass spectrometry | pt |
dc.type | bookPart | pt |
degois.publication.firstPage | 329 | pt |
degois.publication.lastPage | 334 | pt |
degois.publication.location | Coimbra | pt |
degois.publication.title | Acta Medicinae Legalis et Socialis | pt |
dc.peerreviewed | yes | pt |
dc.identifier.doi | 10.14195/978-989-26-0173-1_56 | pt |
dc.date.embargo | 2010-01-01 | * |
uc.date.periodoEmbargo | 0 | pt |
item.fulltext | Com Texto completo | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | bookPart | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
crisitem.author.dept | Faculty of Medicine | - |
crisitem.author.dept | Faculty of Medicine | - |
crisitem.author.parentdept | University of Coimbra | - |
crisitem.author.parentdept | University of Coimbra | - |
crisitem.author.orcid | 0000-0003-1495-9362 | - |
crisitem.author.orcid | 0000-0002-7366-6765 | - |
Appears in Collections: | FMUC Medicina - Livros e Capítulos de Livros |
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File | Description | Size | Format | |
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4937387.pdf | 947.83 kB | Adobe PDF | View/Open |
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This item is licensed under a Creative Commons License