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Título: | The RNA-Binding Protein hnRNP K Mediates the Effect of BDNF on Dendritic mRNA Metabolism and Regulates Synaptic NMDA Receptors in Hippocampal Neurons | Autor: | Leal, Graciano Comprido, Diogo Luca, Pasqualino De Morais, Eduardo Manuel Firmo Rodrigues, Luís Mele, Miranda Santos, Ana R. Costa, Rui O. Pinto, Maria Joana Patil, Sudarshan Berentsen, Birgitte Afonso, Pedro M. Carreto, Laura Li, Ka Wan Pinheiro, Paulo S. Almeida, Ramiro D. Santos, Manuel A. S. Bramham, Clive R. Duarte, Carlos B. |
Palavras-chave: | BDNF; local translation; long-term synaptic potentiation; neurotrophins; NMDA receptors; RNA transport | Data: | 2017 | Editora: | Society for Neuroscience | Projeto: | PTDC/SAU-NEU/104297/2008 info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID/NEU/04539/2013 SFRH/BPD/115546/2016 SFRH/BPD/84593/2012 |
Título da revista, periódico, livro ou evento: | eNeuro | Volume: | 4 | Número: | 6 | Resumo: | Brain-derived neurotrophic factor (BDNF) is an important mediator of long-term synaptic potentiation (LTP) in the hippocampus. The local effects of BDNF depend on the activation of translation activity, which requires the delivery of transcripts to the synapse. In this work, we found that neuronal activity regulates the dendritic localization of the RNA-binding protein heterogeneous nuclear ribonucleoprotein K (hnRNP K) in cultured rat hippocampal neurons by stimulating BDNF-Trk signaling. Microarray experiments identified a large number of transcripts that are coimmunoprecipitated with hnRNP K, and about 60% of these transcripts are dissociated from the protein upon stimulation of rat hippocampal neurons with BDNF. In vivo studies also showed a role for TrkB signaling in the dissociation of transcripts from hnRNP K upon high-frequency stimulation (HFS) of medial perforant path-granule cell synapses of male rat dentate gyrus (DG). Furthermore, treatment of rat hippocampal synaptoneurosomes with BDNF decreased the coimmunoprecipitation of hnRNP K with mRNAs coding for glutamate receptor subunits, Ca2+- and calmodulin-dependent protein kinase IIβ (CaMKIIβ) and BDNF. Downregulation of hnRNP K impaired the BDNF-induced enhancement of NMDA receptor (NMDAR)-mediated mEPSC, and similar results were obtained upon inhibition of protein synthesis with cycloheximide. The results demonstrate that BDNF regulates specific populations of hnRNP-associated mRNAs in neuronal dendrites and suggests an important role of hnRNP K in BDNF-dependent forms of synaptic plasticity. | URI: | https://hdl.handle.net/10316/108142 | ISSN: | 2373-2822 | DOI: | 10.1523/ENEURO.0268-17.2017 | Direitos: | openAccess |
Aparece nas coleções: | IIIUC - Artigos em Revistas Internacionais FCTUC Ciências da Vida - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais |
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The-RNAbinding-protein-hnRNP-K-mediates-the-effect-of-BDNF-on-dendritic-mRNA-metabolism-and-regulates-synaptic-NMDA-receptors-in-hippocampal-neuronseNeuro.pdf | 3.62 MB | Adobe PDF | Ver/Abrir |
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