Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10316/5170
Título: | Effect of pH and applied potential on the adsorption of DNA on highly oriented pyrolytic graphite electrodes. Atomic force microscopy surface characterisation | Autor: | Brett, Ana Maria Oliveira Paquim, Ana Maria Chiorcea |
Palavras-chave: | DNA-electrochemical biosensor; Adsorption; MAC mode AFM | Data: | 2003 | Citação: | Electrochemistry Communications. 5:2 (2003) 178-183 | Resumo: | Single-stranded and double-stranded DNA electrochemical biosensors prepared by adsorption during 3 min on HOPG, with or without an applied potential, at pH 5.3 and 7.0, were characterised by MAC mode AFM. During adsorption DNA condenses on the substrate forming complex network films with pores exposing the HOPG surface. The thin films formed in pH 5.3 acetate buffer always presented a better coverage of the HOPG surface with DNA molecules than films formed in pH 7.0 phosphate buffer. The application of a positive potential of 300 mV during adsorption enhanced the robustness and stability of the DNA films with the formation of bigger network holes and a more condensed and compact self-assembled DNA lattice. The knowledge of the morphology of adsorbed DNA on electrode surfaces explains non-specific adsorption on the electrode surface and can be used to improve and develop DNA-electrochemical biosensors. | URI: | https://hdl.handle.net/10316/5170 | DOI: | 10.1016/S1388-2481(03)00014-6 | Direitos: | openAccess |
Aparece nas coleções: | FCTUC Química - Artigos em Revistas Internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
filed0b2f62c4ccf44e69c0f2b708da3377d.pdf | 281.88 kB | Adobe PDF | Ver/Abrir |
Citações SCOPUSTM
46
Visto em 11/nov/2022
Citações WEB OF SCIENCETM
50
40
Visto em 2/mai/2023
Visualizações de página 50
440
Visto em 25/set/2024
Downloads 50
384
Visto em 25/set/2024
Google ScholarTM
Verificar
Altmetric
Altmetric
Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.