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https://hdl.handle.net/10316/113627
Title: | Effect of Thymbra capitata (L.) Cav. on Inflammation, Senescence and Cell Migration | Authors: | Silva, Jorge M. Alves Pedreiro, Sónia Cavaleiro, Carlos Cruz, M. Teresa Figueirinha, Artur Salgueiro, Lígia |
Keywords: | aging; phytochemical composition; inflammatory mediators; essential oil; hydrodistillation residual water | Issue Date: | 17-Apr-2023 | Publisher: | MDPI | Project: | UIDB/04539/2020 UIDP/04539/2020 LA/P/0058/2020 |
Serial title, monograph or event: | Nutrients | Volume: | 15 | Issue: | 8 | Abstract: | Aromatic plants are reported to display pharmacological properties, including anti-aging. This work aims to disclose the anti-aging effect of the essential oil (EO) of Thymbra capitata (L.) Cav., an aromatic and medicinal plant widely used as a spice, as well as of the hydrodistillation residual water (HRW), a discarded by-product of EO hydrodistillation. The phytochemical characterization of EO and HRW was assessed by GC-MS and HPLC-PDA-ESI-MSn, respectively. The DPPH, ABTS, and FRAP assays were used to disclose the antioxidant properties. The anti-inflammatory potential was evaluated using lipopolysaccharide-stimulated macrophages by assessing NO production, iNOS, and pro-IL-1β protein levels. Cell migration was evaluated using the scratch wound assay, and the etoposide-induced senescence was used to assess the modulation of senescence. The EO is mainly characterized by carvacrol, while the HRW is predominantly characterized by rosmarinic acid. The HRW exerts a stronger antioxidant effect in the DPPH and FRAP assays, whereas the EO was the most active sample in the ABTS assay. Both extracts reduce NO, iNOS, and pro-IL-1β. The EO has no effect on cell migration and presents anti-senescence effects. In opposition, HRW reduces cell migration and induces cellular senescence. Overall, our study highlights interesting pharmacological properties for both extracts, EO being of interest as an anti-aging ingredient and HRW relevant in cancer therapy. | URI: | https://hdl.handle.net/10316/113627 | ISSN: | 2072-6643 | DOI: | 10.3390/nu15081930 | Rights: | openAccess |
Appears in Collections: | FCTUC Eng.Química - Artigos em Revistas Internacionais FMUC Medicina - Artigos em Revistas Internacionais I&D CNC - Artigos em Revistas Internacionais I&D CERES - Artigos em Revistas Internacionais FFUC- Artigos em Revistas Internacionais I&D ICBR - Artigos em Revistas Internacionais |
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